2019-03-12 · The cells were counted in five random fields per well. For BMSCs grafts evaluation, rats were sacrificed at 7 days after transplantation and the femoral trochlea were harvested. Frozen sections were stained by anti-Caspase-3 antibody (ab13847, Abcam, UK) at 4 °C overnight, and anti-rabbit immunoassay kit (GeneTech, China) at 37 °C for 1 h.

Antibody Information. Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3, anti- active Caspase 3. Clonality: polyclonal antibody. Comments: validation status unknown, seller recommendations provided in 2012: Flow Cytometry; Immunocytochemistry; Immunohistochemistry; Western Blot; Flow Cytometry,

Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN. UniProt Code History. A8K5M2, D3DP53, P42574, Q96AN1 Catalog Number ab13847; Quantity 100 UG; Applications Western blot; Concentration 1 mg/ml; Conjugate/Tag Unconjugated; Antigen Caspase-3; Isotype IgG; Host Rabbit; Antibody Type Primary; Gene Name CASP3; NCBI Full Gene Name caspase 3; NCBI Gene Aliases CPP32, CPP32B, SCA-1; Clonality Polyclonal; Validation KnockOut Validated Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application.

IP0280). Inflammation drives atherosclerotic plaque progression and rupture, and is a compelling therapeutic target. Consequently, attenuating inflammation by reducing local macrophage accumulation is an appealing approach. This can potentially be accomplished by either blocking blood monocyte recruitment to the plaque or increasing macrophage apoptosis and emigration. Because macrophage proliferation 2018-07-18 · Loss of function following injury to the CNS is worsened by secondary degeneration of neurons and glia surrounding the injury and is initiated by oxidative damage. However, it is not yet known which cellular populations and structures are most vulnerable 2020-08-10 · Background This study investigated the role of fibrinogen-like protein 1 (FGL1) in regulating gefitinib resistance of PC9/GR non-small cell lung cancer (NSCLC). Methods The effect of different concentrations of gefitinib on cell proliferation were evaluated using the CCK-8 assay.

2018-11-14

dilution (caspase3, ab13847; caspase9, ab32539; p65, ab16502; IKKα, ab178870; IκBα, ab109300; Abcam, Cambridge, MA, USA) overnight. After washing the cells with PBS, we incubated the cells with fluorescent secondary antibody dilution (Sigma-Aldrich, St. Louis, MO, USA) for 1 hour in the dark.

Anti-Caspase 9 Antibody, active (cleaved) form Chemicon®, from rabbit; find Sigma-Aldrich-AB3629 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.

2020-10-21 2020-05-24 Chronic cerebral hypoperfusion (CCH) is a main cause of vascular dementia and is also an etiological factor of neurological diseases and mental disorders. However, few treatments are available for CCH, and new medications are needed. In the present study, we employed a rat model of CCH that was based on bilateral common carotid artery occlusion and investigated the therapeutic effects of 2019-03-12 ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Protein Names.

Apoptosis plays a key role in the pathogenesis of Parkinson disease (PD). Active caspase-3, which is a proapoptotic factor, has been shown to reduce cardiac contractility, causing cardiac dysfunction in many pathological diseases. Reduced cardiac contractility Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 Anti-GATA4 [6H10] (Mouse monoclonal) Thermo Fisher Scientific Membranes were blocked with 5 % non-fat milk in Tris-buffered saline with Tween (TBST) for 2 h and incubated with primary anti-bodies in 5 % milk/TBST at 4 °C overnight, including anti-Bax (Abcam, cat# ab32503, 1:1000), anti-Bcl-2 (Abcam, cat# ab196495, 1:500), anti-Caspase-3 (Abcam, cat# ab13847, 1:500), anti-AKT (Abcam, cat# ab179463, 1 p65 or IKZF1 overexpression inhibits the proliferation of MDA-MB-231 cells. p65 and IKZF1 were overexpressed in MDA-MB-231 cells to analyze whether p65 and IKZF1 had an influence on MDA-MB-231 cell proliferation. Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems. We report an approach to manufacturing interleukin-10 (IL-10)–loaded EVs (IL-10+ EVs) by engineering macrophages for treating ischemic acute kidney injury (AKI).
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#ab32124, Abcam, 1:1000 dilution), as mentioned in Characterization of engineered exosomes. caspase3 (ab49822, Abcam), total caspase3 (ab13847, Abcam), E-cadherin (CST, Danvers, MA, USA) and β-actin (cat. no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C.

IF. Anti-CD3 (. Alexa Fluor®. 647-conjugated).
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dilution (caspase3, ab13847; caspase9, ab32539; p65, ab16502; IKKα, ab178870; IκBα, ab109300; Abcam, Cambridge, MA, USA) overnight. After washing the cells with PBS, we incubated the cells with fluorescent secondary antibody dilution (Sigma-Aldrich, St. Louis, MO, USA) for 1 hour in the dark. We then washed the cells with PBS

ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Antibody Information. Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3, anti- active Caspase 3. Clonality: polyclonal antibody. Comments: validation status unknown, seller recommendations provided in 2012: Flow Cytometry; Immunocytochemistry; Immunohistochemistry; Western Blot; Flow Cytometry, The following primary antibodies were used: Anti-vimentin (cat.